Whitney Group - Synthetic Photosynthesis - bioengineering enzymes to adjust carbon fixation

Our research focuses on the development and use of synthetic biology tools to scrutinize the biology of the biospheres most abundant protein, the photosynthetic CO2-fixing enzyme Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). The research provides training in molecular biology, protein engineering (directed evolution), enzyme kinetics, biochemistry, plastome transformation, tissue culture, measuring leaf photosynthesis and plant growth.

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Selected publications

Some recent research papers

Sharwood, R, Crous, K, Whitney, S.M et al (2017) 'Linking photosynthesis and leaf N allocation under future elevated CO2 and climate warming in Eucalyptus globulus', Journal of Experimental Botany, vol. 68, 1157-1167.

Heureux, A, Young, J, Whitney, S.M et al (2017) 'The role of Rubisco kinetics and pyrenoid morphology in shaping the CCM of haptophyte microalgae', Journal of Experimental Botany, vol. Online, doi: 10.1093/jxb/erx179.

Sharwood, R, Sonawane, B, Ghannoum, O, Whitney, S.M (2016) 'Improved analysis of C4 and C3 photosynthesis via refined in vitro assays of their carbon fixation biochemistry', Journal of Experimental Botany, vol. 67, no. 10, pp. 3137-3148.

Young, J, Heureux, A, Sharwood, R, Whitney, S.M (2016) 'Large variation in the Rubisco kinetics of diatoms reveals diversity among their carbon-concentrating mechanisms',  Journal of Experimental Botany, vol. 67, no. 11, pp. 3445-3456.

Sharwood, R, Ghannoum, O & Whitney, S.M (2016) 'Prospects for improving CO2 fixation in C3-crops through understanding C4-Rubisco biogenesis and catalytic diversity', Current Opinion in Plant Biology, vol. 31, pp. 135-142.

Sharwood, R, Ghannoum, O, Kapralov, M, Gunn, L, Whitney, S.M (2016) 'Temperature responses of Rubisco from Paniceae grasses provide opportunities for improving C3 photosynthesis', Nature Plants, vol. 2, no. 16186, pp. 1-9.

Wilson, R, Alonso, H & Whitney, S.M (2016) 'Evolving Methanococcoides burtonii archaeal Rubisco for improved photosynthesis and plant growth', Scientific Reports, vol. 6, Article 22284.

Whitney, S.M, Birch, R, Kelso, C et al (2015) 'Improving recombinant Rubisco biogenesis, plant photosynthesis and growth by coexpressing its ancillary RAF1 chaperone', Proceedings of the National Academy of Sciences of the United States of America, vol. 112, no. 11, pp. 3564-3569.

Some reviews

Bracher, A, Whitney, S.M, Hartl, F et al (2017) 'Biogenesis and metabolic maintenance of Rubisco', Annual Review of Plant Biology, vol. 68, pp. 29-60.

Wilson, R & Whitney, S.M (2015) 'Photosynthesis: Getting it together for CO2 fixation', Nature Plants, vol. 1, pp. 15130.

Parry, M, Andralojc, P, Scales, J, Salvucci, ME, Carmo-Silva, E, Alonso, H, Whitney, SM. (2013) 'Rubisco activity and regulation as targets for crop improvement', Journal of Experimental Botany, vol. 64, 717-730

Whitney S.M., Houtz R.L and Alonso H (2011) Advancing our understanding and capacity to engineer nature’s CO2 sequestering enzyme, Rubisco.  Plant Physiology 155, 27-35

Andrews, T.J. and Whitney, S.M. (2003) Manipulating Rubisco in the chloroplasts of higher plants. Archives of Biochemistry and Biophysics 414: 159-169

Synthetic biology in E. coli - Directed evolution of Rubisco

Wilson, R & Whitney, S.M (2017) 'Improving CO2 Fixation by Enhancing Rubisco Performance', in Miguel Alcalde (ed.), Directed Enzyme Evolution: Advances and Applications, Springer, Spain, pp. 101-126

Wilson, R, Alonso, H & Whitney, S.M (2016) 'Evolving Methanococcoides burtonii archaeal Rubisco for improved photosynthesis and plant growth', Scientific Reports, vol. 6, Article 22284

Mueller-Cajar and Whitney S.M. (2008) Directing the evolution of Rubisco and Rubisco activase - first impressions of a new tool for photosynthesis research. Photosynthesis Research, 98, 667-675

Mueller-Cajar and Whitney S.M. (2008) Evolving improved Synechococcus Rubisco functional production in Escherichia coli. Biochemical Journal,414, 201-214

Mueller-Cajar O, Morell M, Whitney S.M. (2007) Directed evolution of Rubisco in E. coli reveals a specificity-determining hydrogen bond in the Form II enzyme. Biochemistry, 46, 14067-74

Greene D.N, Whitney S.M, Matsumura I. (2007) Artificially evolved Synechococcus PCC6301 Rubisco variants exhibit improvements in folding and catalytic efficiency. Biochemical Journal, 404, 517-24

Synthetic biology in chloroplasts - engineering of Rubisco and Rubisco activase

von Caemmerer, S, Tazoe, Y, Evans, J, Whitney S.M (2014) 'Exploiting transplastomically modified Rubisco to rapidly measure natural diversity in its carbon isotope discrimination using tuneable diode laser spectroscopy', Journal of Experimental Botany, 65, pp. 3759-3767.

Whitney, S & Sharwood, R (2014), 'Plastid transformation for Rubisco engineering and protocols for assessing expression.', in Pal Maliga (ed.), Chloroplast Biotechnology. Methods and Protocols, Humana Press, New York, pp. 245-262.

Sharwood, R & Whitney, S (2014) 'Correlating Rubisco catalytic and sequence diversity within C3 plants with changes in atmospheric CO2 concentrations', Plant Cell and Environment, 37, 1981-1984.

Pengelly, J, Forster, B, von Caemmerer, S, Badger M, Price GD & Whitney SM (2014) 'Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts', Journal of Experimental Botany, 65, 3071-3080.

Galmes, J, Perdomo, J, Flexas, J and Whitney, SM. (2013) 'Photosynthetic characterization of Rubisco transplantomic lines reveals alterations on photochemistry and mesophyll conductance', Photosynthesis Research, 115, 153-166.

Price, G, Pengelly, J, Forster, B, von Caemmerer, S, Badger, MR, Price, GD and Whitney, SM. (2013) 'The cyanobacterial CCM as a source of genes for improving photosynthetic CO2 fixation in crop species', Journal of Experimental Botany, 64, 753-768.

Whitney, S.M Sharwood, R.E, Orr, D White, S.J, Alonso, H and Galmes, J (2011) Isoleucine 309 acts as a C4 catalytic switch that increases ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylation rate in Flaveria. Proceedings of the National Academy of Sciences 108, 14688-14693.

Blayney, M, Whitney, S.M and Beck, J (2011) NanoESI mass spectrometry of Rubisco and Rubisco activase structures and their interactions with nucleotides and sugar phosphates'. Journal of the American Society for Mass Spectrometry 22, 1588-1601.

Alonso H, Blayney M.J, Beck J.L and Whitney S.M (2009) Substrate induced assembly of Methanococcoides burtonii D-Ribulose-1,5-Bisphosphate carboxylase/oxygenase dimers into decamers. Journal of Biological Chemistry 284, 33876-82

Whitney S.M and Sharwood R.E (2008) Construction of a tobacco master line to improve Rubisco engineering in chloroplasts. Journal of Experimental Botany 59, 1909-1921

Sharwood R.E, von Caemmerer S, Maliga P and Whitney S.M. (2008) The catalytic properties of hybrid Rubisco comprising tobacco small and sunflower large subunits mirror the kinetically equivalent source Rubiscos and can support tobacco growth. Plant Physiology 146, 83-96

Whitney, S.M and Sharwood R.E (2007). Linked Rubisco subunits can assemble into functional oligomers without impeding catalytic performance. Journal of Biological Chemistry 282, 3809-3818

Rubisco bioengineering in chloroplasts using synthetic biology– where it all began

Whitney, S.M. and Andrews, T.J. (2003) Photosynthesis and growth of tobacco with a substituted bacterial Rubisco mirror the properties of the introduced enzyme Plant Physiology 133, 287-294

Whitney, S.M. and Andrews, T.J (2001) Plastome-encoded bacterial ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) supports photosynthesis and growth in tobacco. Proceeding of the National Academy of Sciences 98: 14738-14743.

Whitney, S. M. and T. J. Andrews (2001) The gene for the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit relocated to the plastid genome of tobacco directs the synthesis of small subunits that assemble into Rubisco. Plant Cell 13: 193-205.

Whitney, S.M., von Caemmerer, S., Hudson, G.S. and Andrews, T.J. (1999) Directed mutation of the Rubisco large subunit of tobacco influences photorespiration and growth. Plant Physiology, 121, 579-588.

All publications

Seeing the light: Better plants, better future

Seeing the light: Better plants, better future

Story | Wednesday 3 February 2021
Increasing food production is one of the greatest challenges of the 21st century. The Australian Academy of Science has produced a video featuring researchers who are from the ARC Centre of Excellence for Translational Photosynthesis and the ANU Research School of Biology.

Back to the future: new study could lead to bumper crops

Story | Tuesday 14 July 2020
Research led by scientists at The Australian National University (ANU) could lead to major improvements in crop production.
Yu Zhou PhD student in lab

Yu Zhou, RSB PhD student - International Day of Women and Girls in Science

Story | Tuesday 11 February 2020
Read how Yu Zhou's PhD research is improving photosynthesis to feed the world: the red algae story.

Modifying Rubisco

Story | Tuesday 13 June 2017
Photosynthesis is an essential biological process that depends on the activity of the enzyme Rubisco which catalyses carbon fixation. Rubisco is slow, inefficient and cannot accurately distinguish between CO2 and O2. ANU researchers have been trying to improve the efficiency of Rubisco in crop plants.

Common grass could help boost food security

Story | Tuesday 29 November 2016
Panic grasses could hold the secret to increasing the yields of cereal crops and help feed the world with increasing temperature extremes and a population of nearly 10 billion people by 2050.

Australian funded Plant Growth Facility opens in the Philippines

Story | Friday 19 February 2016
The Loyd T Evans Growth Facility (PGF), named after John Evan's father, was officially opened on the Philippine campus of the International Rice Research Institute on 21 January 2016.

Spurring production of a sluggish enzyme for crop yields

Story | Tuesday 3 March 2015
“The enzyme Rubisco has baffled scientists for over 50 years, as it is thousands of times less efficient at its job than most other enzymes,” said Dr Spencer Whitney.

RSB Student Conference 2014

Story | Thursday 4 December 2014

New research centre set to secure food for the future

Story | Saturday 25 October 2014
A research centre exploring new technologies for improving crop yields to secure food supply has been launched at the Research School of Biology.