The enzyme Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (RuBisCO) is responsible for the entry of atmospheric carbon into the biosphere during photosynthesis. Despite this key role, RuBisCO maintains several biochemical shortcomings, making it an attractive target for laboratory protein engineering.

Site-specific C-to-U RNA editing is a hallmark of plant organelle transcript maturation. Up to thousands of specific cytidines are converted into uridines in plant chloroplasts and mitochondria, with no evidence of editing activity in the cytosol.

Photorespiration (PR) is the pathway that detoxifies the product of the oxygenation reaction of Rubisco. It has been hypothesized that in dynamic light environments, PR provides a photoprotective function by serving as a sink for excess ATP and/or reducing equivalents.

The tiny, self-contained genetic system of the chloroplast (or plastid) in the green alga Chlamydomonas reinhardtii is well suited for genetic engineering and has recently seen a surge in the deployment of synthetic biology approaches.

In my talk, I’d like to introduce two of my postdoctoral studies where I explored the independent expression of two genes in tobacco: Rubisco activase (RCA) and a spider silk gene, Major ampullate spidroin 1 (MaSp1).

Talk followed by panel discussion about future directions in Plant Science.