Abstract -  Accurate estimation of gas exchange parameters has always been a fundamental aim of plant physiologists; from the more general assumptions and calculations presented by Moss and Rawlins (1963) up to the widely used model introduced by von Caemmerer and Farquhar (1981). Plant physiologists are always aiming for more precise and reliable measurements to interpret physiological traits or genetic variations. In this constant demand for increased precision, the previously trivial assumptions made in the calculations become important sources of error as greater precision is sought.

I examined H₂O vapour and CO₂ pathways and dynamics in leaves during gas exchange, focusing on three main aspects of it: (1) cuticular conductance to H₂O and CO₂; (2) adaxial and abaxial leaf gas exchange; and (3) leaf internal CO₂ concentration. The objective was to determine the impact of these factors on the calculation of gas exchange parameters, generating a mathematical theory to quantify them in more detail, aiming for a theoretical approach with practical implications, rather than a solely descriptive outcome.

During my thesis I used data from gas exchange measurements made using various devices, combined in different experimental setups, to obtain a quantitative analysis of leaf gas exchange. Experiments were performed on a variety of plant species to generate a robust dataset to support a theoretical approach and validate the proposed method. As for many studies in plant physiology, the internal CO₂ concentration is a crucial direct or indirect factor for interpreting the results; so I devoted special attention to the estimation of internal CO₂ concentration. My theoretical approach resulted in a more accurate calculation of leaf gas exchange values and increased the exactitude of the estimation of internal leaf CO₂ concentration.

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Biography - PhD student in Farquhar Group

Who you are, a little about yourself

I studied Agronomic Engineering at the University of Chile and a Masters in Soil and Water management also at the University of Chile. I worked in the winery sector for several years, focusing my work on water management mostly.

A bit about your research and why it is important

My research is focused on improving our understanding of leaf gas exchange dynamics and the calculations of gas exchange parameters, through generating mathematical theory and models, aiming for a theoretical approach with practical implications.

What drives you to research plant science?

I have always been amazed by the concept that plants (photosynthetic organisms) are the main source of energy for all the organisms in the earth. That is why I enjoying studying the interaction between water and the soil-plant-atmosphere continuum that allows terrestrial plants to succeed in non-aquatic environments.

Your experience with RSB over the past few years

I have met a lot of great people during the years I have been in RSB. RSB has a very supporting and friendly environment, which has created a very enjoyable experience during my PhD.