PS Webinar Series: Building a Bacterial CO2 Concentrating Mechanism

Many photosynthetic organisms employ a CO2 concentrating mechanism (CCM) to increase the rate of CO2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global photosynthesis, yet it remains unclear which genes and proteins are necessary for a CCM to function.

schedule Date & time
Date/time
12 Aug 2020 12:00pm
person Speaker

Speakers

Dr Avi Flamholz, NSF Fellow at UC Berkeley and Postdoc at Caltech, California, US
next_week Event series
contact_support Contact

Content navigation

Description

Abstract -Many photosynthetic organisms employ a CO2 concentrating mechanism (CCM) to increase the rate of CO2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global photosynthesis, yet it remains unclear which genes and proteins are necessary for a CCM to function. Based on a genome-wide screen in an H. neapolitanus, a proteobacterium that depends on a CCM for autotrophic growth, our lab identified a cluster of 20 genes that, in principle, contains all the activities required for a bacterial-type CCM. I will describe how we constructed a functional CCM in a non-native host by expressing these 20 genes in an engineered E. coli strain. A functional CCM enabled E. coli to grow by fixing CO2 from ambient air into biomass, with growth depending on known CCM components in a predictable manner. Bacterial CCMs are therefore genetically compact and readily transplanted, rationalizing their presence in diverse bacteria as the outcome of horizontal transfer. Reconstitution also enabled genetic experiments refining our understanding of the CCM, thereby laying the groundwork for deeper study and engineering of the cell biology supporting CO2 assimilation in diverse organisms. More broadly, in the spirit of the famous Feynman quote “What I cannot create, I do not understand,” I hope to suggest that working at the interface of “synthetic biology” and “basic research” is an exciting and intellectually profitable way to pursue scientific questions.

Biography - Avi recently graduated from the Molecular and Cell Biology PhD program at UC Berkeley, where he was an NSF fellow in the lab of David Savage and studying the CO2 concentrating mechanism. Avi gets excited when physical and mathematical principles help explain the behavior and evolution of cells, organisms and ecosystems. He's motivated by environmental questions and hopeful that principled approaches to biological engineering will ameliorate the environmental degradation that humanity has collectively wrought. Avi is starting a postdoc in the labs of Dianne Newman and Rob Phillips at Caltech in the fall.

 

Location

When: Aug 12, 2020 12:00 PM Canberra, Melbourne, Sydney

Topic: Building a Bacterial CO2 Concentrating Mechanism

Please click the link below to join the webinar:
https://anu.zoom.us/j/95055885983?pwd=QmxiVTNYaW1hTUZUZHRSamxlUzhYQT09

Webinar ID: 950 5588 5983
Passcode: 777205

Or iPhone one-tap :
    Australia: +61731853730,,95055885983#,,,,,,0#,,777205#  or +61861193900,,95055885983#,,,,,,0#,,777205#
Or Telephone:
    Dial(for higher quality, dial a number based on your current location):
        Australia: +61 731 853 730  or +61 861 193 900  or +61 8 7150 1149  or +61 2 8015 6011  or +61 3 7018 2005

    International numbers available: https://anu.zoom.us/u/abyX0ndIzs

Or an H.323/SIP room system:
    H.323: 113.197.7.15 or 113.197.7.150
    Meeting ID: 950 5588 5983
    Passcode: 777205
    SIP: 95055885983@113.197.7.15 or 95055885983@113.197.7.150
    Passcode: 777205

Upcoming events in this series

Rowan Sage
7 May 2025 | 12pm

C4 photosynthesis is one of the most prolific complex traits in the biosphere, having independently evolved over 70 times in flowering plants. Understanding C4 evolution is providing insights into how evolution builds complex life forms that can transform the biosphere.

View the event