Whitney Lab - Synthetic Photosynthesis - bioengineering enzymes to adjust carbon fixation

Algal version of Rusico expressed in Tobacco

Our research focuses on utilising novel biotechnological tools to scrutinize the biology of the biospheres most abundant protein, the photosynthetic CO2-fixing enzyme Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). The research provides training in molecular biology, protein engineering (directed evolution), enzyme kinetics, biochemistry, plastome transformation, tissue culture, measuring leaf photosynthesis and plant growth.

Members

Leader

Spencer Whitney

Spencer Whitney
Spencer undertook his BSc (Botany, Biochemistry and Chemistry) honours and PhD at James Cook University of North Queensland...

PhD Student

Postdoctoral Scientist

Research Assistant

Research Officer

Technical Officer

Visiting Fellow

Publications

Selected publications

General papers on Rubsico

  • Whitney S.M., Houtz R.L and Alonso H (2011) Advancing our understanding and capacity to engineer nature’s CO2 sequestering enzyme, Rubisco.  Plant Physiology 155, 27-35
  • Andrews, T.J. and Whitney, S.M. (2003) Manipulating rubisco in the chloroplasts of higher plants. Archives of Biochemistry and Biophysics 414: 159-169

Directed evolution of Rubisco

  • Mueller-Cajar and Whitney S.M. (2008) Directing the evolution of Rubisco and Rubisco activase - first impressions of a new tool for photosynthesis research. Photosynthesis Research, 98, 667-675
  • Mueller-Cajar and Whitney S.M. (2008) Evolving improved Synechococcus Rubisco functional production in Escherichia coli. Biochemical Journal,414, 201-214
  • Mueller-Cajar O, Morell M, Whitney S.M. (2007) Directed evolution of Rubisco in E. coli reveals a specificity-determining hydrogen bond in the Form II enzyme. Biochemistry, 46, 14067-74
  • Greene D.N, Whitney S.M, Matsumura I. (2007) Artificially evolved Synechococcus PCC6301 Rubisco variants exhibit improvements in folding and catalytic efficiency. Biochemical Journal, 404, 517-24

Engineering of Rubisco and Rubisco activase

  • Whitney, S.M Sharwood, R.E, Orr, D White, S.J, Alonso, H and Galmes, J (2011) Isoleucine 309 acts as a C4 catalytic switch that increases ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) carboxylation rate in Flaveria. Proceedings of the National Academy of Sciences 108, 14688-14693.
  • Blayney, M, Whitney, S.M and Beck, J 2(011) NanoESI mass spectrometry of Rubisco and Rubisco activase structures and their interactions with nucleotides and sgar phosphates'. Journal of the American Society for Mass Spectrometry 22, 1588-1601.
  • Alonso H, Blayney M.J, Beck J.L and Whitney S.M (2009) Substrate induced assembly of Methanococcoides burtonii D-Ribulose-1,5-Bisphosphate carboxylase/oxygenase dimers into decamers. Journal of Biological Chemistry 284, 33876-82
  • Whitney S.M and Sharwood R.E (2008) Construction of a tobacco master line to improve Rubisco engineering in chloroplasts. Journal of Experimental Botany 59, 1909-1921
  • Sharwood R.E, von Caemmerer S, Maliga P and Whitney S.M. (2008) The catalytic properties of hybrid Rubisco comprising tobacco small and sunflower large subunits mirror the kinetically equivalent source Rubiscos and can support tobacco growth. Plant Physiology 146, 83-96
  • Whitney, S.M and Sharwood R.E (2007). Linked Rubisco subunits can assemble into functional oligomers without impeding catalytic performance. Journal of Biological Chemistry 282, 3809-3818
  • Whitney, S.M. and Andrews, T.J. (2003) Photosynthesis and growth of tobacco with a substituted bacterial Rubisco mirror the properties of the introduced enzyme Plant Physiology 133, 287-294
  • Whitney, S.M. and Andrews, T.J (2001) Plastome-encoded bacterial ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) supports photosynthesis and growth in tobacco. Proceeding of the National Academy of Sciences 98: 14738-14743.
  • Whitney, S. M. and T. J. Andrews (2001) The gene for the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit relocated to the plastid genome of tobacco directs the synthesis of small subunits that assemble into Rubisco. Plant Cell 13: 193-205.

All publications

News & events

News

29
Nov
2016
Panic grasses could hold the secret to increasing the yields of cereal crops and help feed the world with increasing temperature extremes and a population of nearly 10 billion people by 2050.
Lloyd T. Evans Plant Growth Facility (PGF), IRRI, Los Baños, Philippines
19
Feb
2016
The Loyd T Evans Growth Facility (PGF), named after John Evan's father, was officially opened on the Philippine campus of the International Rice Research Institute on 21 January 2016.
Rob Sharwood
09
Nov
2015
RSB Plant Scientist Rob Sharwood has been awarded a T
Maxim Kapralov (L) and Spencer Whitney. Image: Charles Tambiah / ARC-CoETP
03
Mar
2015
“The enzyme Rubisco has baffled scientists for over 50 years, as it is thousands of times less efficient at its job than most other enzymes,” said Dr Spencer Whitney.

Pages

Highlights

Elena

Elena Martin Avila

I am currently working in the Whitney Lab (aka Rubisco Lab), as part of the ARC Centre of Excellence for Translational Photosynthesis. I became more interested in issues related to sustainable agricultural development and food security, and that’s why I came to do a Postdoc here at ANU.
Elena

Elena Martin Avila in Samoa

Whitney group post doc Elena Martin Avila will be in Samoa for 7 months this year (2017), to work as a Tissue Culture Officer for the Samoan Ministry of Agriculture and Fisheries, in the Crops Research Division.

Modifying Rubisco

Photosynthesis is an essential biological process that depends on the activity of the enzyme Rubisco which catalyses carbon fixation. Rubisco is slow, inefficient and cannot accurately distinguish between CO2 and O2.

Updated:  25 July 2017/Responsible Officer:  Director RSB/Page Contact:  Webmaster RSB