Transcription factors (TFs) play an important role in regulating transcription and gene expression. Transcriptional regulation is controlled by both general and sequence-specific TFs, as well as their interaction with chromatin factors. In collaboration with Dr. Qi Dai’s lab at Stockholm University, we are investigating transcriptional gene regulation by cell-type specific TFs, BEN-solo family, controlling neural cell fate specification. We are using both genome-wide deep-sequencing approaches (ChIP-seq, ChIP-nexus, ATAC-seq, Pro-seq, RNA-seq, Hi-C), combined with genetics and biochemistry experiments to study their function in the Notch signaling pathway and their interaction with chromatin factors.
Related papers for this project from Dr. Qi Dai's group:
Dai Q, Ren A, Westholm JO, Duan H, Patel DJ and Lai EC. Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family. Genes & Development. 2015; 29(1): 48-62. PMID: 25561495
Dai Q, Andreu-Agullo C, Insolera R, Wong LC, Shi SH and Lai EC. BEND6 is a nuclear antagonist of Notch signaling during self-renewal of neural stem cells. Development. 2013; 140 (9) :1892-902. PMID: 23571214
Dai Q*, Ren A*, Westholm JO, Serganov AA, Patel DJ and Lai EC. The BEN domain is a novel sequence-specific DNA binding domain conserved in neural transcriptional repressors. Genes and Development. 2013;27(6):602-14. PMID: 23468431. (* co-first author).