Thousands of human genes produce multiple distinct mRNA isoforms through alternative polyadenylation (APA), which has significant effects on neural development and function. However the regulator factors of APA is not well understood. We propose the potential role of RNA-binding protein Elav families in regulating APA events in the central nervous system. This project will involve analyzing various deep-sequencing datasets generated by collaborators at Professor Eric Lai's Lab — Elav family CLIP-seq, 3’-seq, RNA- seq, and Nascent RNA-seq — to understand the regulation and function of the 3’ ends of genes, in particular in the nervous system.
Recent papers from this project:
Sanfilippo P., Wen J., Shenker S., and Eric C. Lai. (2017). Landscape and evolution of tissue-specific alternative polyadenylation across Drosophila species. (In review)
Sanfilippo, P., P. Miura, and E. C. Lai (2017). Genome-wide profiling of the 3’ ends of polyadenylated RNAs. Methods S1046-2023(17) 30137-8.