Roles of RNA-binding protein Elav family in regulating alternative polyadenylation in the central nervous system
Thousands of human genes produce multiple distinct mRNA isoforms through alternative polyadenylation (APA), which has significant effects on neural development and function. However the regulator factors of APA is not well understood. We propose the potential role of RNA-binding protein Elav families in regulating APA events in the central nervous system. This project will involve analyzing various deep-sequencing datasets generated by collaborators — Elav family CLIP-seq, 3’-seq, RNA- seq, and Nascent RNA-seq — to understand the regulation and function of the 3’ ends of genes, in particular in the nervous system.