SMALPs: life-belts for membrane proteins

Dr Naomi Pollock - University of Birmingham

Description

Though crucial to life and highly relevant to many diseases, membrane proteins offer fascination and frustration in equal measure to the biochemist. Proteins that have evolved to exist within the complex chemistry of the lipid bilayer rarely prosper when removed from it, yet purification of proteins is a cornerstone of biochemistry. Research in our laboratory focuses on solving this dilemma. 
We use an amphipathic copolymer of 2:1 styrene: maleic acid (SMA) that has been shown to directly solubilize most biological membranes, to stabilize membrane proteins, and to be amenable to biophysical, functional and structural analyses. Crucially, SMA solubilizes lipids rather than proteins, forming SMA lipid particles (SMALPs) in which membrane protein are retained in their native lipid environment and exhibit excellent functional and structural stability.
As our understanding of SMA and SMALPs deepens, we have had success in the purification and study of more than 50 membrane proteins with a variety of architectures and sizes, both beta barrels and alpha helical proteins from 40 to 400 kDa in size. Here I present an overview of this work, and show the ongoing method developments that we have pursued to broaden the applications of SMA and SMALPs to membrane protein research.

Biography
Naomi completed her undergraduate degree at the University of Oxford, and stayed on to do a PhD studying human ATP-binding cassette (ABC) transporters under the supervision of Richard Callaghan. Her first post-doctoral position at the University of Manchester continued this theme of ABC protein work, with a focus on the structure and function of CFTR. Naomi now works at the University of Birmingham developing a novel method for membrane protein purification using amphipathic polymers of styrene and maleic acid.

Date & time

1–2pm 10 October 2017

Location

Slatyer Seminar Room, D.A. Brown Building (Bldg 47), Daley Road, ANU

Speakers

Dr Naomi Pollock School of Biosciences, University of Birmingham. Host: Richard Callaghan

Contacts

 Rowena Martin
 61 (0) 2 6197 0051

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