PS Seminar Series: PhD exit Seminar - Dissecting molecular mechanisms of disease in the wheat pathogen, Parastagonospora nodorum

Description

Abstract - Parastagonospora nodorum is a wheat specific pathogen that causes annual losses to the Australian wheat industry in excess of $100 million AUD. This necrotrophic fungus kills the host tissue generating necrotic lesions within which fruiting bodies develop, spreading spores and continuing the disease cycle. This polycyclic infection cycle leads to field epidemics resulting in the losses to growers. Sporulation and virulence are the two crucial aspects for disease development in the P. nodorum-wheat pathosystem and form the basis of this project.

A forward genetics approach was employed to discover novel mechanisms by which P. nodorum facilitates infection on wheat. A library of random P. nodorum insertion mutants was generated, and subsequently screened for gain and loss of virulence phenotypes on non-susceptible and susceptible wheat cultivars. From a library of 950 transformants seven displayed a consistent avirulent phenotype on the susceptible wheat cultivar, and one displayed a partial gain of virulence on the non-susceptible cultivar. To identify the disrupted loci leading to avirulence, genomes of the seven avirulent P. nodorum strains were sequenced elucidating a Catechol-1,2-dioxygenase and a Copper dependent amine oxidase.

To complement the virulence investigation, a combined transcriptomics and metabolomics approach was employed to decipher sporulation in this pathogen. This is of particular interest as the canonical sporulation pathways in other, model fungi, were previously shown to be not applicable in P. nodorum. A differential gene analysis of fungal material collected at critical developmental time points identified several key genes involved in initiating a sporulation cascade. Notably, a WetA homolog was identified, along with an uncharacterised Aquaporin-like protein and a Pr1-like protein. Reverse genetics has been employed, and is in the final stages of determining the role of these candidate genes in P. nodorum during fungal development.

Biography - PhD Student in Peter Slomon's lab

Date & time

3.30–4.30pm 10 November 2017

Location

Gould Seminar room (235), Gould Building 116, Daley Road, ANU

Speakers

Oliver Mead, PhD Student, Solomon lab, RSB, ANU

Contacts

 Gagan Bhardwaj
 02 6125 9395

Updated:  22 November 2017/Responsible Officer:  Director RSB/Page Contact:  Webmaster RSB