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Jones Lab - Disease resistance

Teaching | Research | Research opportunities | Lab members | Publications | Grants

Assoc Prof David Jones

Building 134,
Research School of Biology,
The Australian National University,
Acton, ACT 0200
T: 61254192
E: david.jones@anu.edu.au

 Biography:

David Jones obtained his B.Sc. (Hons) and PhD at the Department of Genetics, University of Adelaide working on the genetics of the interaction between flax and the flax rust fungus Melampsora lini. He was then awarded a Charles John Everard Postdoctoral Fellowship in the Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide where he worked on the biological control of crown gall. His work led to the first commercial release of a genetically modified bacterium, subsequently marketed as NoGall. He was then offered a postdoctoral fellowship in The Sainsbury Laboratory, John Innes Centre, Norwich, where he worked on the genetic and molecular basis of the interaction between tomato and the leaf mould fungus Cladopsorium fulvum. His work led to the isolation of the tomato Cf-9 gene, one of the first plant disease resistance genes cloned. In 1996, he returned to Australia to set up his own lab in the Research School of Biological Sciences (now the Research School of Biology) at The Australian National University, where he continues work on the molecular basis of disease resistance in tomato (leaf mould and Fusarium wilt resistance) and flax (flax rust resistance).

Teaching

BIOL3177/6177 Advances in Molecular Plant Science (Co-convenor)
BIOL3174 Special Projects
BIOL3106/6106 Biosecurity (Convenor)

Research interests

Current projects

Subcellular localisation of plant disease resistance proteins

We have used green fluorescent protein fusions to localise flax resistance proteins conferring resistance to flax rust.        Show more detail...

Activation of plant disease resistance proteins

We have used site-directed mutagenesis and agroinfiltration to investigate molecular function in the tomato Cf-9 protein conferring leaf mould resistance.        Show more detail...

Mapping and functional characterisation of the tomato I-7 gene for resistance to Fusarium wilt

This project aims to characterise I-7 resistance by microscopic analysis and digital gene expression analysis. We also aim to map I-7 and develop PCR-based markers for marker-assisted breeding of I-7.        Show more detail...

Identification and functional characterisation of the tomato I-3 gene for resistance to Fusarium wilt.

This project aims to identify the I-3 gene and to characterise the I-3 protein. We also aim to characterise I-3 resistance by microscopic analysis and digital gene expression analysis.        Show more detail...

Fungal effector uptake into plant cells

Pathogens secrete effector proteins that are transported into the cytoplasm of infected plant cells        Show more detail...

Student research opportunities

Identification and functional characterisation of flax rust Avr genes

This project will combine next generation sequencing, bioinformatics and classical genetics to identify new Avr genes, and use an array of molecular and cell biology techniques to analyse Avr gene function.        Show more detail...
Projects suitable for Honours and PhD students are also available in each of the other areas of current research described above and enquiries from prospective students are welcome.

Lab members

  • Claire Anderson (Postdoctoral Fellow)
  • Ann-Maree Catanzariti (ARC Postdoctoral Fellow)
  • Huong Do (PhD student)
  • Yvonne Gonzalez-Cendales (PhD student)
  • David Jones (Lab Leader)
  • Kevin Choon Yang Tee (PhD student)

» Go to lab directory

Publications

Selected publications

Takemoto, D., Rafiqi, M., Hurley, U., Lawrence, G.J., Bernoux, M., Hardham, A.R., Ellis, J.G., Dodds, P.N. and Jones, D.A. 2012. N-terminal motifs in some plant disease resistance proteins function in membrane attachment and contribute to disease resistance. Molecular Plant-Microbe Interactions 25:379-392.

Rafiqi, M., Gan, P.H.P., Ravensdale, M., Lawrence, G.J., Ellis, J.G., Jones, D.A., Hardham, A.R., and Dodds, P.N. 2010. Internalization of flax rust avirulence proteins into flax and tobacco cells can occur in the absence of the pathogen. Plant Cell 22:2017-2032.

Wulff, B.B.H., Chakrabarti, A., and Jones, D.A. 2009. Recognitional specificity and evolution in the tomato-Cladosporium fulvum pathosystem. Molecular Plant-Microbe Interactions 22:1191-1202.

Chakrabarti, A., Panter, S.N., Harrison, K., Jones, J.D.G., and Jones, D.A. 2009. Regions of the Cf-9B disease resistance protein able to cause spontaneous necrosis in Nicotiana benthamiana lie within the region controlling pathogen recognition in tomato. Molecular Plant-Microbe Interactions 22:1214-1226.

Wulff, B.B., Heese, A., Tomlinson-Buhot, L., Jones, D.A., de la Peña, M., and Jones, J.D.G. 2009. The major specificity-determining amino acids of the tomato Cf-9 disease resistance protein are at hypervariable solvent-exposed positions in the central leucine-rich repeats. Molecular Plant-Microbe Interactions 22:1203-1213

Lim, G.T.T., Wang, G.P., Hemming, M.N., McGrath, D.J., and Jones, D.A. 2008. High resolution genetic and physical mapping of the I-3 region of tomato chromosome 7 reveals almost continuous microsynteny with grape chromosome 12 but interspersed microsynteny with duplications on Arabidopsis chromosomes 1, 2 and 3. Theoretical and Applied Genetics 118:57-75.

Barker, C.L., Talbot, S.J., Jones, J.D.G., and Jones D.A. 2006. A tomato mutant that shows stunting, wilting, progressive necrosis and constitutive expression of defence genes contains a recombinant Hcr9 gene encoding an autoactive protein. Plant Journal 46:369-384.

Barker, C.L.,  Baillie, B.K., Hammond-Kosack, K.E., Jones, J.D.G., and Jones, D.A. 2006. Dominant-negative interference with defence signalling by truncation mutations of the tomato Cf-9 disease resistance gene. Plant Journal  46:385-399.

Takemoto, D., and Jones, D.A. 2005. Membrane release and destabilization of Arabidopsis RIN4 following cleavage by Pseudomonas syringae AvrRpt2. Molecular Plant-Microbe Interactions 18: 1258-1268.

Benghezal, M., Wasteneys, G.O., and Jones, D.A. 2000. The C-terminal dilysine motif confers endoplasmic reticulum localization to type I membrane proteins in plants. Plant Cell 12:1179-1201.

Jones, D.A., Thomas, C.M., Hammond-Kosack, K.E., Balint-Kurti, P.J., and Jones, J.D.G. 1994. Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. Science 266:789-793.

All publications

Click here to see a full list of publications on the ISI website...

Recent grants

Dr David Jones and Dr Peter Dodds (CSIRO Plant Industry)
ARC Discovery 2012-2014
Isolation and functional characterisation of a pathogen meta effector able to inhibit detection of multiple disease effectors by resistant plants
$340,000

Dr Ann-Maree Catanzariti and Dr Martijn Rep (University of Amsterdam)
ARC Discovery - APD 2010-2012
Pathogen recognition and plant defence activation by a novel Fusarium wilt resistance protein from tomato
$330,000

Prof. Adrienne Hardham, Dr David Jones, Dr Peter Dodds (CSIRO Plant Industry) and Dr Jeff Ellis (CSIRO Plant Industry)
ARC Discovery 2010-2012
Role of fungal secreted proteins as plant disease effectors
$330,000

Dr David Jones and Mr Des McGrath (Queensland Department of Primary Industries and Fisheries)
ARC Linkage - PhD Studentship 2010-2012
Protecting tomato crops from Fusarium wilt through the efficient application of new genetic resources.
$80,000



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