Teaching
BIOL3161: Genomics and its Applications (Convenor)
BIOL1004: Molecular and Cell Biology Molecular and Cell Biology (lectures on plants and human health)
Our group has received four University medals, three outstanding honours theses awards and two awards for excellence in teaching and supervision in the past eight years (see below).
Research interests
SIGNALING BETWEEN THE CHLOROPLAST AND NUCLEUS
The overarching theme of our research is to determine the controls and regulators of communication between the chloroplast and nucleus. This includes discovery of genes and metabolites involved in epigenetics, RNA metabolism, chloroplast-nuclear signaling, carotenoid biosynthesis, photosynthesis and drought. The research  program is an integral component of the ARC Centre of Excellence in Plant Energy Biology (see below). The Centre offers Honours scholarships ($6K) and topup scholarships for PhD students ($7K), in addition to well funded research, conference travel and graduate training programs. 1. Signaling during chloroplast development.
• First demonstration of a role for the cytoskeleton and a novel cytoskeleton-associated protein in chloroplast biogenesis (Albrecht et al 2010, Plant Cell). • Elucidation of a mechanism for photosystem assembly that requires lipid vesicles and a protein disulphide isomerase that folds light harvesting and other photosystem proteins (Tanz et al. 2011, Plant Journal).
 2. Identification of the first signal to move from the chloroplast to the nucleus
• We are studying the mechanisms by which plants perceive and respond to drought and excess light. Recently, we uncovered a molecule that moves between the chloroplast and nucleus to regulate gene expression. The key publication is Estavillo et al 2011, Plant Cell, which builds upon Marin et al 2011 Plos One, Wilson et al. 2009 Plant J. and Rossel et al 2006.
3. Intersection of drought and high light signaling. • Demonstration of a role for the ER and an ER processing protein in chloroplast-nuclear communication, specifically in response to drought (Che et al. 2010, Science Signaling) • Commercial outputs. The knowledge of the SAL-PAP retrograde pathway has been published (Rossel et al. 2006, PCE Wilson et al. 2009, Plant Journal), patented and is being applied to the development of drought tolerant wheat in collaboration with CSIRO and GRDC and in Canola with Pacific Seeds via an ARC Linkage grant. Also see Gonzalo Estavillo's web page. 4. Epigenetic Regulation of Carotenoid Biosynthesis: Carotenoids, such as beta-carotene, are essential for photosynthesis, dietary vitamin A and as antioxidants offer protection against certain cancers and age-related blindness. We made the first identification of an epigenetic regulator of carotenoid biosynthesis. Communication between the chloroplast and nucleus occurs in both directions and a seminal finding was the identification of a histone methylation transferase that regulates specific enzymes required for carotenoid biosynthesis, flowering time and hormone synthesis in the chloroplast. Future goals are to elucidate the epigenetic controls and identify novel carotenoid-derived retrograde signals produced from carotenoids that regulate nuclear gene expression and plant development. See Cazzonelli et al. 2009 Plant Cell and Cazzonelli web page 5. Leaf to leaf systemic signalling A leaf exposed to full sunlight will transmit a signal to shaded leaves enable a systemic acquired acc  limation (SAA) response. The nature and regulation of SAA is under investigation. 6. Photosynthesis and photoprotection We are investigating the safety valves plants used to protect against oxidative damage induced during photosynthesis. This program is a combination of biochemical genetics and physiology using Arabidopsis and avocado.
7. Collaborative projects: Several projects are undertaken by students based in CSIRO. For example, there is a project relating to cereal colour and nutritional content of wheat with Dr Crispen Howitt. A key to our research is teamwork and applying cutting edge molecular and biochemical techniques in combination with whole plant physiology to build a picture from gene to crop.The approach utilizes deep sequencing, microarrays, promoter-reporter gene fusions, HPLC and GC-MS to identify novel genes and signalling pathways in Arabidopsis (related to Canola), wheat, avocado and canola. Some sample projects are listed below. More are available upon request. Also check out others in our group who have their own web sites.
Current projects
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Carotenoids and Plant Development
This proposal will unravel the regulatory mechanisms controlling carotenoid composition as well as plant growth.
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Understanding chloroplast/nuclear signaling and response pathways which determine photosynthetic capacity
Aim: To gain new insights into chloroplast/nuclear signaling pathways using in mutants where normal protein expression is perturbed by RNAi down regulation.
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Chloroplast development
The plant organelles responsible for the plants ability to perform photosynthesis, the chloroplasts, are essential for plant growth and vitality.
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Student research opportunities
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Mechanisms by which a chromatin-modifying gene targets gene expression and promotes memory
Epignetics is a memory forming process in plants that involves chromatin modifications. This proposal will unravel the regulatory mechanisms controlling carotenoid composition and epigenetic events in plants.
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Identification of novel carotenoid-derived signalling pathways affecting plant development
Carotenoids are colourful plant pigments. This proposal aims to identify new carotenoid signaling pathways regulating development in response to environmental change.
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The molecular nature of metabolic feedback regulation by metabolite-binding RNA riboswitches
Metabolite binding RNA riboswitches are rare in plants. This proposal will invesigate a putative metabolite-binding riboswitch in Arabidopsis.
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Regulation of carotenoid composition, growth habit and root-mycorrhizal symbiosis in tomato
Carotenoids play important roles in mediating root-mycorrhizal symbiosis as well as defining growth habit and development. This proposal will investigate how perturbing carotenoid compositon in tomato affects such processes.
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Protein modification in chloroplast
A light sensitive mutant, snowy cotyledon 4, has been isolated with a mutation in a CAAX-proteinase function
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SCO Protein interaction partners
Several novel proteins have been identified in the mutant analysis of snowy cotyledon mutants, but mostly nothing is known about their function
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Gain of function mutants
In aim to unravel the regulatory network of chloroplast biogenesis second site mutants of snowy cotyledon 2 and snowy cotyledon 3 have been identified
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Lab members
- Veronica Albrecht (Research Associate)
- Andrew Bowerman (PhD student)
- Melanie Carmody (PhD student)
- Chris Cazzonelli (Research Associate)
- Kai Chan (PhD student)
- Derek Collinge (Laboratory Manager)
- Lee Constable (Lab Preparation)
- Peter Crisp (PhD student)
- Matthew Dunn (Honours Student)
- Gonzalo Estavillo (Research Associate)
- Matthew (Matt) Gordon (PhD student)
- Rebecca Harper (Education Officer)
- Arun Kumar (Research Associate)
- Shashikanth Marri (Postdoctoral Fellow)
- Nazia Nisar (PhD student)
- Barry Osmond (Visiting Fellow)
- Su Yin Phua (PhD student)
- Barry Pogson (Lab Leader)
- John Rivers (Laboratory Technician)
- Tepsuda Rungrat (PhD student)
- Estee Tee (Lab Preparation)
- Shelley Verma (Laboratory Technician)
» Go to lab directory
Lab awards and achievements
- Seven articles from the lab in top 1% of Plant and Animal Sciences with respect to citations in past 10 years
- Kai Xun Chan: University Medal and India Australia Association Prize for the Most Outstanding Honours thesis in the College of Medicine, Biology and Environment Prize, 2010
- Gonzalo Estavillo: ACT Tall Poppy Early Career Researcher Award, ALTC NAtional Teaching Award, ASPS Teaching Award, Vice-Chancellors Excellence in Teaching Award, 2010-2011
- Chris Cazzonelli: Goldacre Medal, awarded by the Australian Society for Plant Physiology, 2010
- Peter Crisp: India Australia Association Prize for the Most Outstanding Honours thesis in the College of Medicine, Biology and Environment Prize, 2009
- Xiao Hui Chew, Awarded the University medal, 2008 for an outstanding honours thesis
- Abby Cuttriss: ASPS and Functional Plant Biology Best Paper Award for Young Scientists to Cuttriss et al. 2007
- Jan Bart Rossel: Cover of Plant Cell and Article selected by Editors for review in “In brief” for Rossel et al. 2007
- Britta Förster: Rebiez Foundation Award for Best Paper in the area of Chloroplast Biology to Förster, Mathesius and Pogson, 2006
- Jan Bart Rossel: ARC APD Fellowship, 2006
- Nick Woo: Awarded the University medal, 2005 for an outstanding Honours thesis
- Barry Pogson: Vice Chancellor’s Award for Excellence in Supervision of research students and postdoctoral fellows. One award is made annually at the ANU,2005
- Alexandra Chubb: Awarded the University medal and the ASBMB prize for an outstanding honours thesis, 2004
- Philippa Walter: Awarded the ASBMB prize for an outstanding honours thesis, 2003
- Hyoungshin Park: Park et al Plant Cell selected by Editors for review in “In this Issue”, 2002
- Barry Pogson: Fenner Medal, awarded by the Australian Academy of Science for distinguished research in plant and animal sciences by scientists 40 years or under, 2001
- Barry Pogson: Goldacre Medal, awarded by the Australian Society for Plant Physiology on the merit of original research by an early career scientist, 2001
Publications
Selected publications
Select Publications from a total of over 40 in the past five years (see link for complete list)
2012 pubs- Tanz, SK, Kilian, J, Johnsson, C, Apel, K, Small, ID, Harter, K, Wanke, K, Pogson, BJ and Albrecht, V (2012) The SCO2 protein disulphide isomerase is required for thylakoid biogenesis and interacts with LCHB1 chlorophyll a/b binding proteins which affects chlorophyll biosynthesis in Arabidopsis seedlings. Plant Journal (published online Dec 2, 2011; DOI: 10.1111/j.1365-313X.2011.04833.x)
- Nichol, CJ, Pieruschka, R, Takayama, K, Förster, B, Kolber, Z, Rascher, U, Grace, J, Robinson, SA, Pogson, BJ, and Osmond, CB (2012) Canopy conundrums: exploring the envelope of reversible down-regulation of photosynthesis in the outer and inner canopy to scale observations from the leaf to the landscape. Functional Plant Biology (in press).
2011 pubs- Estavillo, GM, Crisp, PA, Pornsiriwong, W, Wirtz, M, Collinge, D, Carrie, C, Giraud, E, Whelan, J, David, P, Javot, H, Brearley, C, Hell, R, Marin, E and Pogson, BJ (2011) Evidence for a SAL1-PAP Chloroplast Retrograde Pathway that Functions in Drought and High Light Signaling in Arabidopsis. Plant Cell 23: 3992-4012
- Cuttriss, AJ, Cazzonelli, CI, Wurtzel, ET and Pogson, BJ (2011) Vitamin A (carotenoids). IN Biosynthesis of vitamins in Plants, Eds F. Rébeillé and R. Douce, Advances in Botanical Research
- Hirsch J, Misson J, Crisp PA, David P, Bayle V, Estavillo GM, Javot H, Chiarenza S, Mallory AC, Maizel A, Declerck M, Pogson BJ, Vaucheret H, Crespi M, Desnos T, Thibaud M-C, Nussaume L, Marin E (2011) A Novel fry1 Allele Reveals the Existence of a Mutant Phenotype Unrelated to 5'->3' Exoribonuclease (XRN) Activities in Arabidopsis thaliana Roots. PLoS ONE 6(2): e16724
- Förster, B, Pogson, BJ and Osmond CB (2011) Lutein from de-epoxidation of lutein epoxide replaces zeaxanthin to sustain an enhanced capacity for non-photochemical chlorophyll fluorescence quenching in avocado shade leaves in the dark. Plant Physiol. 156: 393-403.
- Pogson, BJ, and Albrecht, V (2011) Genetic dissection of chloroplast biogenesis and development: an overview. Plant Physiol. 155:1545-1551.
- *Woo, NS, *Gordon, MJ, Graham, SR, Rossel, JB, Badger, MR and Pogson, BJ (2011) A mutation in the purine biosynthetic enzyme ATASE2 impacts high light signalling and acclimation responses in green and chlorotic sectors of Arabidopsis leaves. Functional Plant Biology 38:401-419.
- Albrecht V, Estavillo GM, Cuttriss AJ and Pogson BJ (2011) Identifying chloroplast biogenesis and signalling mutants in Arabidopsis thaliana. Methods in Molecular Biology 684:257-72.
select 2010 pubs- Albrecht, V, Šimková, K, Carrie, C, Delannoy, E, Giraud, E Whelan, J, Small, I Apel, K, Badger, M and Pogson, BJ (2010) The cytoskeleton and the peroxisomal-targeted SNOWY COTYLEDON 3 protein are required for chloroplast development in Arabidopsis. Plant Cell 22(10):3423-38.
- Che, P, Bussell, JD Zhou, W Estavillo, G, Pogson, BJ, Smith SM (2010) Signaling from the Endoplasmic Reticulum Activates Brassinosteroid Signaling and Promotes Acclimation to Stress in Arabidopsis. Science Signaling 3 (141), ra69. [DOI: 10.1126/scisignal.2001140].
- Cazzonelli CI and Pogson BJ. (2010) A balance between source and sink: regulation of carotenoid composition in plants. Trends in Plant Science 15(5): 266-274
select 2009 pubs- Cazzonelli, CI, Cuttriss, AJ, Cossetto, SB, Pye, W, Whelan, J, Finnegan, J, Turnbull, C and Pogson, BJ (2009) Epigenetic regulation of carotenoid composition in Arabidopsis. Plant Cell 21: 39-53.
- Wilson, PB, Estavillo, GM, Field, KJ, Carroll, AJ, Pornsiriwong, W, Howell, KA, Lake, JA, Millar, AH, von Caemmerer, S, Pogson, BJ (2009) The nucleotidase/phosphatase, SAL1, is a negative regulator of drought tolerance in Arabidopsis. Plant Journal 58: 299-317
- Delannoy, E, Le Ret, M, Faivre-Nitschke, SE, Estavillo, GM, Bergdoll, M, Taylor, NL, Pogson, BJ, Small, ID, Imbault, P, and Gualberto, JM (2009) Arabidopsis tRNA adenosine deaminase (TADA) is responsible for editing the wobble nucleotide of chloroplast tRNAArg(ACG), a modification essential for efficient chloroplast translation. Plant Cell 21: 2058-2071
- Millar, AH, Carrie, C, Pogson, BJ and Whelan J (2009) Exploring the function:location nexus - using multiple lines of evidence in defining the subcellular location of plant proteins Plant Cell 21: 1625-1631.
- Howitt CA, Cavanagh C, Bowerman AF, Cazzonelli CI, Rampling L, Mimica JL, and Pogson BJ. (2009) Alternative splicing, activation of cryptic exons and amino acid substitutions in carotenoid biosynthetic genes are associated with lutein accumulation in wheat endosperm. Functional and Integrative Genomics 9:363-376
Select earlier publications
- Rossel, JB Wilson, PB, Hussain, D, Woo, NS, Gordon, MJ, Mewett, OP, Howell, KA, Whelan, J, Kazan, K, Pogson, BJ (2007) Systemic and Intracellular Response to Photooxidative stress in Arabidopsis Plant Cell 19: 4091-4110. (ISI highly cited)
- Pogson, B.J., Woo, NS, Förster B and Small, ID (2008) Plastid signalling to the nucleus and beyond. Trends in Plant Science 13 (11): 602-609 (ISI highly cited)
- DellaPenna, D. and Pogson, B.J. (2006) Vitamin synthesis in plants: tocopherols and carotenoids. Annual Review of Plant Biology 57: 711-738 (ISI highly cited)
- Millgate, A.G., Pogson, B.J., Wilson, I.W., Zenk, M.H., Gerlach, W.L., Fist, A.J. and Larkin, P.J. (2004) A morphine-free mutant of opium poppy - changing the poppy analgesic industry. Nature 431:413-414.
- Park H., Kreunen S.S., Cuttriss, A.J., DellaPenna, D., Pogson B.J. (2002) Identification of the carotenoid isomerase provides insight into carotenoid biosynthesis, prolamellar body formation and photomorphogenesis. Plant Cell 14: 321-332. (ISI highly cited)
- Rossel J.B., Wilson I.W., and Pogson B.J. (2002) Global changes in gene expression in response to high light in Arabidopsis. Plant Physiol. 130:1109-1120 (ISI highly cited)
- Pogson B.J., Niyogi K.K., Björkman O., and DellaPenna D. (1998) Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants. Proc. Natl. Acad. Sci. USA 95: 13324-29.
- Pogson B.J., McDonald K., Truong M., Britton G., and DellaPenna D. (1996) Arabidopsis carotenoid mutants demonstrate lutein is not essential for photosynthesis in higher plants. Plant Cell 8: 1627-1639.
All publications
 Click here to see a full list of publications on the ISI website...
Recent grants
- Badger, Von Caemmerer, Price, Goodnow, Pogson “Improving plant productivity and human health using next generation biotechnology approaches” ARC SuperScience Fellowships $556.8K
- Pogson, Estavillo and Howitt “The generation of wheat cultivars with improved drought tolerance and agronomic traits” Grains Research Development Council $300K
- Shannon, Dennis, McNevin, Oakeshott, Allison, Cazzonelli, Cook, Crisp, Easteal, Foley, Georges, Goodnow, Gordon, Graves, Hardham, Keogh, Linde, Mahalingam, Peakall, Pogson, Saint, Spriggs, Tremethick, Yung “A massively parallel genome analysis facility for the ACT region” ARC LIEF $1.6M
- Whelan, Smith, Badger, Small, Millar, Day, Pogson, "ARC Centre of Excellence in Plant Energy Biology (CPEB)", $2.5M per annum.
Links
The ARC Centre of Excellence in Plant Energy Biology is a group of students, postdocs, research asstiants and chief investigators at ANU, University of Western Australia and Flinders University. The group leaders are Badger, Day, Millar, Pogson, Small, Whelan. The Centre offers Honours scholarships ($6K) and topup scholarships for PhD students ($7K), in addition to well funded research, conference travel and graduate training programs. See http://www.plantenergy.uwa.edu.au/ for more details, news and research opportunities.
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